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KMID : 0370220210650010041
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Yakhak Hoeji
2021 Volume.65 No. 1 p.41 ~ p.45
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Cuttlebone Shows Anti-inflammatory Activity Via Suppression of NF-κB Activation in LPS-induced RAW 264.7 Macrophages
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Lee Geum-Seon
Kang Tae-Jin
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Abstract
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Our previous study reported that cuttlebone (CB) extract shows wound healing activity and enhances cellmigration. In the present study, we examined the anti-inflammatory effect of CB in RAW 264.7 cells activated withlipopolysaccharide (LPS) for induction of inflammation. The expression of inflammatory mediators, including proinflammatorycytokines, was measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptionpolymerase chain reaction (RT-PCR) tests. The results showed that CB suppressed nitric oxide (NO) production inmacrophages stimulated with LPS. Production of pro-inflammatory cytokines, such as tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6, decreased in LPS-induced RAW 264.7 cells after treatment with CB in a dose-dependentmanner. Additionally, gene expression was inhibited by CB in a dose-dependent manner in LPS-stimulated macrophages.
Furthermore, CB inhibited nuclear factor kappa B (NF-κB) p65 activation in macrophages activated with LPS. Therefore,these results suggest that CB exerts its anti-inflammatory effects via regulation of the NF-κB signaling pathway.
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KEYWORD
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cuttlebone, inflammation, macrophages, cytokines, NF-κB
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